rh spp1 Search Results


gene exp rhbg hs00220735 m1  (Thermo Fisher)
85
Thermo Fisher gene exp rhbg hs00220735 m1
Gene Exp Rhbg Hs00220735 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rh+spp1/pmc04392232__gutjnl___2013___306228___s2-4-199--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp rhbg hs00220735 m1 - by Bioz Stars, 2026-07
85/100 stars
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rh spp1  (R&D Systems)
95
R&D Systems rh spp1
(A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average <t>SPP1</t> expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.
Rh Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rh+spp1/bio_rxiv__2025__06__12__656421-327-8-16?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
rh spp1 - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
carhsp1 nm 001278260 human tagged orf clone  (OriGene)
N/A
CARHSP1 GFP tagged Human calcium regulated heat stable protein 1 24kDa CARHSP1 transcript variant 3
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carhsp1 nm 001278263 human tagged orf clone  (OriGene)
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CARHSP1 GFP tagged Human calcium regulated heat stable protein 1 24kDa CARHSP1 transcript variant 6
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carhsp1 rnai  (Novus Biologicals)
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carhsp1 rat shrna plasmid  (OriGene)
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Carhsp1 Rat 4 unique 29mer shRNA constructs in lentiviral GFP vector
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carhsp1 nm 014316 human tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles CARHSP1 mGFP tagged Human calcium regulated heat stable protein 1 24kDa CARHSP1 transcript variant 1 200ul 10 7 TU mL
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carhsp1 human shrna plasmid kit  (OriGene)
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CARHSP1 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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carhsp1 antibody (hrp)  (biorbyt)
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Rabbit polyclonal antibody against CARHSP1 conjugated to HRP Isotype Note: IgG Host Note: Rabbit Conjugation Note: HRP Reactivity Note: Human Application Note: ELISA
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carhsp1 nm 001278265 human untagged clone  (OriGene)
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CARHSP1 untagged Human calcium regulated heat stable protein 1 24kDa CARHSP1 transcript variant 8
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carhsp1 mouse qpcr primer pair  (OriGene)
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qSTAR qPCR primer pairs against Mus musculus gene Carhsp1
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carhsp1 antibody  (Bethyl)
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Rabbit anti-CARHSP1 Antibody, Affinity Purified
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Image Search Results


(A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average SPP1 expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.

Journal: bioRxiv

Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

doi: 10.1101/2025.06.12.656421

Figure Lengend Snippet: (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average SPP1 expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.

Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

Techniques: Expressing, Marker, Derivative Assay, Recombinant, Knockdown, Invasion Assay, Transformation Assay

Western blot showing EMT markers in UOK-127 cells treated with 50ng/mL recombinant SPP1 at varying timepoints.

Journal: bioRxiv

Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

doi: 10.1101/2025.06.12.656421

Figure Lengend Snippet: Western blot showing EMT markers in UOK-127 cells treated with 50ng/mL recombinant SPP1 at varying timepoints.

Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

Techniques: Western Blot, Recombinant

Western blot showing SPP1 expression in THP-1 derived M2-like macrophages including control, and treatment with silencing mRNAs siNC, siPC, siSPP1#1, and siSPP1#2.

Journal: bioRxiv

Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

doi: 10.1101/2025.06.12.656421

Figure Lengend Snippet: Western blot showing SPP1 expression in THP-1 derived M2-like macrophages including control, and treatment with silencing mRNAs siNC, siPC, siSPP1#1, and siSPP1#2.

Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

Techniques: Western Blot, Expressing, Derivative Assay, Control

Western blot showing sarcomatoid-specific proteins (Met, CD44, ANXA2) and EMT markers (N-cadherin, Slug, Snail) in UOK-127 cells control, treated with M2-like macrophages, or treated with M2-like macrophages with siRNA knockdown of negative control or SPP1.

Journal: bioRxiv

Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

doi: 10.1101/2025.06.12.656421

Figure Lengend Snippet: Western blot showing sarcomatoid-specific proteins (Met, CD44, ANXA2) and EMT markers (N-cadherin, Slug, Snail) in UOK-127 cells control, treated with M2-like macrophages, or treated with M2-like macrophages with siRNA knockdown of negative control or SPP1.

Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

Techniques: Western Blot, Control, Knockdown, Negative Control

Invasion assay of UOK-127 cell co-cultured with M2 macrophage conditioned media with negative controle (siNC) or silencing of SPP1 (siSPP1) in M2 macrophages.

Journal: bioRxiv

Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

doi: 10.1101/2025.06.12.656421

Figure Lengend Snippet: Invasion assay of UOK-127 cell co-cultured with M2 macrophage conditioned media with negative controle (siNC) or silencing of SPP1 (siSPP1) in M2 macrophages.

Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

Techniques: Invasion Assay, Cell Culture